Insect Research

Fundraising campaign by Nino Angelo Bardon
  • US$535.00
    raised of $12,500.00 goal goal
4% Funded
44 Donors

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Objective:
Objective 1. Develop dsRNA (i.e., RNAi-based gene silencing) and, potentially, other genetic constructs to silence eicosanoid signaling and other immune-related genes in pest insect species, including the squash bug. Subobjective 1A: Clone, express and characterize a recombinant PLA2 from the squash bug, Anasa tristis and other pest insect species and test the influence of silencing PLA2 on standard immune parameters. Subobjective 1B: Identify, clone and silence POX genes in squash bugs, then determine the influence of gene silencing on selected cellular immune parameters. Subobjective 1C: Determine the influence of suppressing insect immune signaling on pest insect life history. Objective 2. Use conventional and molecular methods to develop and optimize western corn rootworm artificial diets. Subobjective 2A. Improve and standardize an artificial diet for rearing the western corn rootworm. Subobjective 2B. Determine molecular and cellular components contributing to WCR survival of Bt intoxication. Objective 3. Improve control of western corn rootworm with the entomopathogenic nematode Heterorhabdidtis bacteriophora by determining the influence of local soil and climate conditions on the survival of the nematode, and the attractiveness of the nematode to the corn root alarm signal (E)-ß-caryophyllene. Objective 4. Establish research-ready cell lines from midgut and other tissues of corn rootworm, fall armyworm, and other pest insect species in support of biotechnology (e.g. RNAi research) products for pest control

Organizer

  • Nino Angelo Bardon

Donors

  • Guest
  • Donated on Feb 25, 2018
$25.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
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44 donors
  • Guest
  • Donated on Feb 25, 2018
$25.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
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  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
  • Guest
  • Donated on Feb 24, 2018
$10.00
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US$535.00
raised of $12,500.00 goal
4% Funded
44 Donors

No more donations are being accepted at this time. Please contact the campaign owner if you would like to discuss further funding opportunities